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pCLL9

規格:

價格:

編號:B226945

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pCLL9

商品貨號

B226945

Designations

pCLL9

Depositors

American Cyanamid Co., JS Feitelson, American Cyanamid Co.

培養基

U.S. Patent

5,002,891

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Vector Information

Size (kb): 9.9099998474121090

Vector: pCLL9 (plasmid)

Construction: pCLL7, pIJ702

Marker(s):ampR,tsrR

Construct size (kb): 9.90999984741211

Features: insert detection: mel
marker(s): ampR
marker(s): tsrR
replicon: pAkij1

Applications

shuttle vector

vector permitting visual detection of recombinants tyrosinase

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--10.2; BglII--10.2; PstI--10.2.

Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.

Cloning into the BglII, SphI, or SstI sites inactivates the tyrosinase gene.

The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped

freeze-dried

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