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當(dāng)前位置: 首頁 > ATCC代理 > GRF-1(1-835) [UTR-02221, WI-9140] B226955
GRF-1(1-835) [UTR-02221, WI-9140]
GRF-1(1-835) [UTR-02221, WI-9140]
規(guī)格:
價格:
編號:B226955
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
GRF-1(1-835) [UTR-02221, WI-9140]
商品貨號
B226955
Designations
GRF-1(1-835) [UTR-02221, WI-9140]
GenBank Number

M73077

培養(yǎng)基
Species
Homo sapiens, human
Applications
in another host, produces protein glucocorticoid receptor DNA binding factor 1
Vector
Construct size (kb): 10.60000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKCR-2
Intact vector size: 5.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BamHI, EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRLF1
Gene name: glucocorticoid receptor DNA binding factor 1
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19, 341.990000 cR
Tissue: breast adenocarcinoma MCF7 cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 4.9
Cross references: DNA Seq. Acc.: M73077
Insert lengths(kb): 4.900000095367432
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: glucocorticoid receptor DNA binding factor 1 [GRLF1]
Target Gene: glucocorticoid receptor DNA binding factor 1
Insert Size (kb)
4.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BglII--6.0, 4.6; EcoRI--5.4, 5.0; EcoRV--5.4, 2.9, 2.3; PvuII--10.5; SacI--6.0, 4.7; XbaI--4.7, 3.1, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.47; BglII--1.67; EcoRV--1.84; NcoI--0.03, 2.14; NdeI--1.73; PstI--0.10, 1.82, 2.76; PvuII--0.55; SacI--0.72; SmaI--1.79; XbaI--2.30.
Contains the complete coding sequence (nt 34-2541) plus 1.5 kb 3' untranslated.
pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.
References

LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621

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