Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
Effect on Host
CPE, refractile rounding and syncytia
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 75-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-5 days
Name of Depositor
ATCC
Source
Clinical patient positive for Human respiratory syncytial virus, Virginia, 2012
