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當(dāng)前位置: 首頁 > ATCC代理 > human CAV cDNA B227606
human CAV cDNA
human CAV cDNA
規(guī)格:
價格:
編號:B227606
品牌:Mingzhoubio

標(biāo)準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
human CAV cDNA
商品貨號
B227606
Designations
human CAV cDNA
GenBank Number

L19297

培養(yǎng)基
Species
Homo sapiens, human
Depositors
WS Sly
Vector
Construct size (kb): 4.079999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CA5
Genomic copy number: unique
Gene name: carbonic anhydrase V
Contains complete coding sequence?: Y
Chromosome: 16
Tissue: liver
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.123
Cross references: DNA Seq. Acc.: L19297
Nucleotides 1-1123 of the insert correspond to
nucleotides 1-1123 of L19297.
Insert lengths(kb): 1.123000025749207
Tissue: liver
Gene product: carbonic anhydrase V [CA5]
Insert Size (kb)
1.123
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BamHI/XhoI--3.0, 1.1; BglI--1.9, 1.3, 0.9; EcoRI--3.6, 0.52; PstI--3.6, 0.4.
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.29; BglI--0.38; EcoRI--0.53; PstI--0.63.
References

Nagao Y, et al. Human mitochondrial carbonic anhydrase: cDNA cloning, expression, subcellular localization, and mapping to chromosome 16. Proc. Natl. Acad. Sci. USA 90: 7623-7627, 1993. PubMed: 8356065

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