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pBG311.bmis [IVI 10090]

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編號:B227730

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pBG311.bmis [IVI 10090]

商品貨號

B227730

Designations

pBG311.bmis [IVI 10090]

GenBank Number

M13151

培養基

Species

Bos taurus, cow

Depositors

Biogen, Inc., RL Cate, Biogen, Inc.

Applications

in another host, produces protein anti-Mullerian hormone (Mullerian inhibiting substance)

Vector

Construct size (kb): 6.535999774932861

Insert

DNA: cDNA

Insert lengths(kb): 2.04200005531311

Tissue: testis

Gene product: anti-Mullerian hormone (Mullerian inhibiting substance) [AMH]

Target Gene: anti-Mullerian hormone (Mullerian inhibiting substance)

Insert Size (kb)

2.042

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): PstI--3.4, 1.5, 0.6, 0.55, 0.5, 0.35; PvuII--6.1, 0.5; HindIII--6.6; BglI--1.8, 1.5, 1.2, 0.6, 0.35, 0.3 (doublet), 0.2.

The insert contains the following restriction sites (nt from the 5' end): PstI--519, 528, 864, 1470, 1549; PvuII--859, 1370; StuI--416.

Constructed by ligating a 376 bp StuI/ApaI fragment of the genomic clone cbmis15 containing 10 bp of 5' flanking sequence, 121 bp of 5' untranslated region, and 245 bp of the coding region (nt 80-456 of the reported sequence),

to a 1666 bp ApaI/HindIII fragment of the cDNA clone pX21 containing the rest of the coding sequence and the 3' untranslated region.

pBG311 is an animal cell expression vector constructed from pAT153 and the SV40 early promoter, the t splice signal, and the T polyadenylation signal from pSV2-dhfr. It includes a 44 bp polylinker.

References

Cate RL, et al. Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685-698, 1986. PubMed: 3754790

Cate RL, Donahoe PK. DNA sequences, recombinant DNA molecules and processes for producing mullerian inhibiting substance-like polypeptides. US Patent 5,047,336 dated Sep 10 1991

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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