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pHPV-16 purified plasmid DNA
pHPV-16 purified plasmid DNA
規(guī)格:
價格:
編號:B228243
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHPV-16 purified plasmid DNA
商品貨號
B228243
Designations
pHPV-16 purified plasmid DNA
GenBank Definition

Human papillomavirus type 16 variant, complete sequence

培養(yǎng)基
GenBank Number

U89348.1

Species
human papilloma virus type 16
Depositors
JE Marich, Syngene, Inc
Vector
Size of Construct (kb): 10.866
Excise insert: BamHI+BglI (insert fragment will include 205 nt of the vector).


Vector: pBluescript SK-
Size of intact vector (kb): 2.960
Markers: ampR
Vector ends: XhoI
Insert
Insert size (kb): 7.904
Genome: human papilloma virus type 16
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: genomic
Insert ends: BamHI
Insert Size (kb)
7.904
Related Products
ATCC 45113
ATCC VR-3240SD
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
200 ng purified plasmid DNA, dried. Rehydrate with TE or water.
Comments
Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site.
Full-length.

The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI - 685 [6818], 1320 [7453].

BamHI+BglI will excise the insert with 205 nucleotides of the vector.

Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6.
Classification
Papovavirus, Papillomavirus, recombinant
References

James E Marich, personal communication

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