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當(dāng)前位置: 首頁 > ATCC代理 > pB34BSM B228773
pB34BSM
pB34BSM
規(guī)格:
價格:
編號:B228773
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pB34BSM
商品貨號
B228773
Designations
pB34BSM
Species
Beet necrotic yellow vein virus
培養(yǎng)基
Depositors
KE Richards
Vector
Construct size (kb): 3.940000057220459
Insert
DNA: cDNA
Insert lengths(kb): 0.7400000095367432
Gene product: genome, partial
Insert Size (kb)
0.740
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PvuII--2.9, 1.0; EcoRI--3.9; BamHI--4.2.
To synthesize antisense RNA in vitro, cut with BamHI and transcribe from vector T3 promoter.
The insert is nt 1-740 of RNA-3 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious.
Classification
Furovirus
References

Bouzoubaa S, et al. Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. J. Gen. Virol. 66: 1553-1564, 1985.

Ken E Richards, personal communication

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