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pPGBC9
pPGBC9
規格:
價格:
編號:B228869
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pPGBC9
商品貨號
B228869
Designations
pPGBC9
Species
Homo sapiens, human
培養基
Depositors
P van den Elsen
Applications
Codominant segregation was observed in 4 unrelated families (49 individuals).
Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs.
Vector
Construct size (kb): 3.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.699999988079071
Tissue: T4+,T8- T-cell clone
Gene product: antigen CD3D, delta polypeptide (TiT3 complex) [T3D]
Alleles: B2, B2, B1, B1, , , , , , , , A1, A2, , , , , , , , A1, A2
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.9; SalI--3.9; PstI--2.5, 1.3; BamHI--2.9, 0.6, 0.5.
TaqI and MspI RFLP alleles are in strong linkage disequilibrium.
Codominant segregation was observed in 4 unrelated families (49 individuals).
The insert contains ATG at 97, TGA at 610, and 89 bp of 3' untranslated sequence including AATAA followed by poly(A) 17 bp downstream.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs.
Enzyme(s) not detecting polymorphism: RsaI, BstEII, EcoRV, BamHI, HindIII, BglII, PvuII, BglI and PstI in at least 6 unrelated individuals.
References

Malhotra U, Concannon P. Human T-cell receptor CD3-delta (CD3D) / MspI DNA polymorphism. Nucleic Acids Res. 17: 2373, 1989. PubMed: 2468134

van den Elsen P, et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312: 413-418, 1984. PubMed: 6095101

Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473

Barbara Weiffenbach, personal communication

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