第一福利网,四色在线,午夜剧场成人

Betacoronavirus 1

規(guī)格:

價格:

編號:B228912

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

Betacoronavirus 1

商品貨號

B228912

Classification

Coronaviridae, Betacoronavirus

Deposited As

Human coronavirus OC43

培養(yǎng)基

Agent

Betacoronavirus 1

Strain

OC43

Common Name

Human coronavirus OC43

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Images

Comments

VR-1558™ is a TC-adapted item derived from virus passaged in mice (ATCC^® VR-759™). Cleaned of mycoplasma with MRA. No antigenic cross reaction with Human coronavirus 229E (ATCC^® VR-740™).

Effect on Host

CPE, vacuolization of cytoplasm and sloughing

Recommended Host

HCT-8 (ATCC^® CCL-244™)

Growth Conditions

Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO₂ atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 33°C in a humidified 5% CO₂ atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.

Name of Depositor

JO Fleming, University of Wisconsin, Madison

Special Collection

ATCC

Chain of Custody

ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian

Source

Man with cold-like illness. Treated to remove mycoplasma at ATCC.

References

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356

Cross References

Nucleotide (GenBank) : NC_005147.1 Human coronavirus OC43, complete genome

B191128：RNA from Betacoronavirus 1, Strain OC43

王經(jīng)理

手機號：17280875617

QQ：1438578920
胡經(jīng)理

手機號：13345964880

QQ：2438244627
周經(jīng)理

手機號：17757487661

QQ：1296385441
于經(jīng)理

手機號：18067160830

QQ：2088210172
沈經(jīng)理

手機號：19548299266

QQ：2662369050
李經(jīng)理

手機號：13626845108

QQ：972239479