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phrosHi6
phrosHi6
規(guī)格:
貨期:
編號(hào):B229050
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
phrosHi6
商品貨號(hào)
B229050
Designations
phrosHi6
Species
Homo sapiens, human
Depositors
K Huebner, L Nagarajan
Vector
Construct size (kb): 8.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 5.5
Tissue: promyelocytic leukemia
Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog [ROS]
Alleles: A1, A2, A1, A2
Insert Size (kb)
5.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 2.3; HindIII--5.5, 2.7; PstI--4.8, 2.1, 1.1; BamHI--5.6, 2.2; AccI--3.7, 2.9, 1.5.
The insert contains SacI (2) and BamHI (1) sites.
The depositor indicates a similar clone has been sequenced in another lab.
This probe spans a large part of the region homologus to v-ros.
Allele A1 was seen in 1/39 cell lines; A2 in 37/39 cell lines, and both present in 1/39. PstI digestion of genomic DNA gives 3 bands of 5.3, 3.2, 1.3 kb in 19/19 cell lines. An additional band of 2.65 kb was detected in 1 of the 19.
Enzyme(s) not detecting polymorphism: BamHI, EcoRV, SstI. The genomic fragment sizes detected are (kb): BamHI--11, 3.15; EcoRV--12; SstI--greater than 30, 16, 1.5.
References

Matsushime H, et al. Human c-ros-1 gene homologous to the v-ros sequence of UR2 sarcoma virus encodes for a transmembrane receptorlike molecule. Mol. Cell. Biol. 6: 3000-3004, 1986. PubMed: 3023956

Nagarajan L, et al. The human c-ros (ROS) gene is located at chromosome region 6q16----6q22. Proc. Natl. Acad. Sci. USA 83: 6568-6572, 1986. PubMed: 3529088

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