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pFBJ-MLV-1
pFBJ-MLV-1
規格:
價格:
編號:B229272
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pFBJ-MLV-1
商品貨號
B229272
Designations
pFBJ-MLV-1
Species
Murine osteosarcoma virus
培養基
Depositors
T Curran
Vector
Construct size (kb): 12.60000038146973
Insert
DNA: genomic
Insert lengths(kb): 8.199999809265137
Tissue: DNA from mouse BALB/c NIH 3T3 cell line
Insert Size (kb)
8.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (kb): HindIII- 1.1- PstI- 2.0- PstI(in the LTR)- 3.6- XhoI- 0.6- SalI- 0.3- SstI- 0.6- HindIII.
Restriction digests of the clone give the following sizes (kb): HindIII--8.2, 4.4; XhoI--12.6.
This contains an entire copy of FBJ-MLV, circularly permuted with a single LTR. Digestion by HindIII and ligation will reconstitute an infectious viral DNA.
Unintegrated supercoiled DNA. MARKER: Ap(r). This clone is circularly permuted with a single LTR. HindIII digestion followed by ligation reconstitutes an infectous viral DNA.
NIH 3T3 cells infected with biologically cloned FBS-MLV (see Curran and Teich, Virology 116: 221-235, 1982)
Unintegrated supercoiled DNA. MARKER: Ap(r). This clone is circularly permuted with a single LTR. Hind III digestion followed by ligation reconstitutes an infectous viral DNA.
References

Curran T, et al. Structure of the FBJ murine osteosarcoma virus genome: Molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells. Mol. Cell. Biol. 3: 914-921, 1983. PubMed: 6306448

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