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pLOI295
pLOI295
規格:
價格:
編號:B229315
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLOI295
商品貨號
B229315
Designations
pLOI295
Species
Zymomonas mobilis (Lindner) Kluyver and van Niel
培養基
Depositors
University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville
Applications
produces protein alcohol dehydrogenase II
produces protein pyruvate decarboxylase
Vector
Construct size (kb): 5.867000102996826
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: alcohol dehydrogenase II [pdc]
Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II
Insert Size (kb)
3.200
Media
1227 plus 2% glucose
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried? E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.7, 3.2; BglI--2.5, 1.3, 1.1, 0.9; HindIII--3.1, 1.7, 0.8, 0.2.
The GenBank accession number for the adh insert is M15394.
Expression appears to be regulated by the lac promoter, and growth is increased under anaerobic conditions.
Constructed by inserting a 1.4 kb EcoRI/SalI fragment from pLOI284 containing the promoterless adh coding sequence into the BamHI site of pLOI276.
The pdc reading frame begins 163 bp downstream of the lac promoter and ends with two stop codons 85 bp upstream of the adhB reading frame. The adhB gene has a single stop codon followed by a palindromic sequence which serves as a terminator.
pLOI284 consists of two DraI fragments of 0.12 and 1.23 kb cloned into the SmaI site of pUC18. The insert starts at the DraI site 402 nt downstream of the 5' DraI site of the reported sequence, with the coding region from nt 30 to 1181 of the insert.
The (5')EcoRI/BamHI (3') fragment contains both genes.
References

Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991

Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063

Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037

Ingram LO, Conway T. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404, 1988.

Ingram LO, et al. Genetic engineering of ethanol production in Escherichia coli. Appl. Environ. Microbiol. 53: 2420-2425, 1987. PubMed: 3322191

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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