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YCpIF10

規格:

價格:

編號:B229538

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YCpIF10

商品貨號

B229538

Designations

YCpIF10

Depositors

RW Davis, PK Foreman

培養基

Biosafety Level

Vector Information

Size (kb): 5.1999998092651370

Vector: YCpIF10 (plasmid)

Promoters: Promoter for expression GAL1

Construction: pUN15

Marker(s):TRP1,ampR

Construct size (kb): 5.199999809265137

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...SalI
centromere: CEN4

Applications

YX-type (expression) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 1.7; BamHI--5.2; EcoRI--5.2.

One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.

Vectors YCpIF10, YCpIF11 and YCpIF12 (ATCC 87087-87089) differ only in the reading frame of the multiple cloning site.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907