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pGIKS-CRE
pGIKS-CRE
規格:
價格:
編號:B229541
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGIKS-CRE
商品貨號
B229541
Designations
pGIKS-CRE
GenBank Number

X03453

培養基
Species
Staphylococcus aureus subsp.aureus bacteriophage P1
Depositors
M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct size (kb): 3.9
Vector end (5'): KpnI
Vector end (3'): SacI
Excise insert: XhoI+SmaI
Promoter giving the sense strand: T3
Promoter giving the antisense strand: T7
Insert
DNA: genomic
Insert lengths(kb): 1.0
Gene product: recombinase, site-specific [cre]
Insert end (5'): KpnI linker
Insert end (3'): SacI
Insert Size (kb)
1.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 485-1516 of Genbank X03453.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; PvuI--0.46; ClaI--0.81.
The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI.
Restriction digests of the clone give the following sizes (kb): SmaI--4.0; SmaI/XhoI--3.0, 1.1; XhoI--4.0.
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