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pIIB1

規格:

價格:

編號:B229594

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pIIB1

商品貨號

B229594

Designations

pIIB1

GenBank Number

X59268

培養基

Species

Homo sapiens, human

Depositors

D Reinberg

Vector

Construct size (kb): 4.619999885559082

Insert

DNA: cDNA

Insert lengths(kb): 1.620000004768372

Tissue: HeLa cell line

Gene product: transcription factor IIB(RNA polymerase II transcription factor IIB) [TCF2B*]

Insert Size (kb)

1.620

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.6; AccI--3.3, 0.86, 0.3; XhoI--3.4, 0.76, 0.46; BamHI--4.6; PvuII--2.4, 1.5, 0.64.

The insert contains the following restriction sites (approximate kb from the 5' cloning site): EcoRI--0.03, 0.04, 0.12; AccI--0.4, 0.64, 0.9; BstXI--0.41; XhoI--0.6, 1.28.

Detects human genomic restriction fragments of the following sizes (approximate kb): BamHI--23; EcoRI--10; HindIII--15;

Contains the complete coding sequence, with 5' and 3' untranslated regions.

and an mRNA in HeLa cells of 1.7 kb.

There are about 40 bp between the 5' reported sequence and the vector cloning site (adjacent to the T3 promoter). This region contains 3 EcoRI sites.

References

Ha I, et al. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature 352: 689-695, 1991. PubMed: 1876184

Danny Reinberg, personal communication

B175637：pIIB1

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