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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pcos1EMBL
pcos1EMBL
規格:
價格:
編號:B229875
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pcos1EMBL
商品貨號
B229875
Designations
pcos1EMBL
Depositors
A Craig
培養基
Biosafety Level
1
Vector Information
Size (kb): 6.3000001907348630
Vector: pcos1EMBL (cosmid)
Promoters: Promoter none
Construction: R6K, pcos4EMBL
Marker(s):kanR,tetR
Construct size (kb): 6.300000190734863
Features: marker(s): kanR, tetR
promoter: none
replicon: R6K
terminator: none
enhancer: none
Applications
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; SalI--6.3.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K origin.
Contains one lambda cos site.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped
freeze-dried
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