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pE25.a [pBS300]
pE25.a [pBS300]
規(guī)格:
價(jià)格:
編號(hào):B229898
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pE25.a [pBS300]
商品貨號(hào)
B229898
Designations
pE25.a [pBS300]
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 3.09500002861023
Insert
DNA: genomic
Insert lengths(kb): 0.3490000069141388
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive [D1Z7]
Insert Size (kb)
0.349
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6.
The first half of the dimer is 85% identical to the sequence of the 1' monomer; the second half is 80% identical to the 2' monomer.
The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof.
References

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Immo E Scheffler, personal communication

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