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pBS-hCD40L-6A9

規(guī)格:

價格:

編號:B229904

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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產品名稱

pBS-hCD40L-6A9

商品貨號

B229904

Designations

pBS-hCD40L-6A9

GenBank Number

L07414

培養(yǎng)基

Species

Homo sapiens, human

Depositors

J Gauchat

Vector

Construct size (kb): 4.059999942779541

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD40LG
Other gene symbols: CD40L
Gene name: CD40 antigen ligand
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q26
Tissue: CD4-positive T-cells
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: L07414

Insert lengths(kb): 1.100000023841858

Tissue: CD4-positive T-cells

Gene product: CD40 antigen ligand [CD40L]

Insert Size (kb)

1.100

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 0.2; HindIII--3.3, 0.7; NcoI--4.0; PvuII--2.6, 1.0, 0.6; EcoRI--3.0, 1.2.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.93; HindIII--0.58; NcoI--0.78; PvuII--0.52.

Contains the complete coding sequence (nt 40-825 of the sequence record).

References

Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552

Jean-Francois Gauchat, personal communication

B223776：pBS-hCD40L-6A9

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