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pLG117R
pLG117R
規(guī)格:
價格:
編號:B229961
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pLG117R
商品貨號
B229961
Designations
pLG117R
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Harvard Univ.
Applications
produces protein interferon, beta 1, fibroblast
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Tissue: fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Tissue: fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references:
Insert lengths(kb): 0.6000000238418579
Tissue: fibroblast
Gene product: interferon, beta 1, fibroblast [IFNB]
Target Gene: interferon, beta 1, fibroblast
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Insert contains an internal PstI site.
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--2.2, 0.6; PstI--1.8, 0.96; BamHI--2.8.
Encodes a 20 kDa protein product in Escherichia coli, corresponding to the expected molecular weight of unglycosylated interferon beta. Demonstrates anti-viral activity (inhibition of cytopathic effect using VSV and human fibroblasts).
Constructed by inserting a lacZ promoter (derivative of lac95 portable promoter) upstream of Bal31-digested pre-interferon beta cDNA.
References

Taniguchi T, et al. Expression of the human fibroblast interferon gene in Escherichia coli. Proc. Natl. Acad. Sci. USA 77: 5230-5233, 1980. PubMed: 6159642

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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