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pAA3H
pAA3H
規格:
價格:詢價
編號:B230000
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAA3H
商品貨號
B230000
Designations
pAA3H
Depositors
A Ahmed
培養基
Biosafety Level
1
Vector Information
Size (kb): 11.5000000000000000
Vector: pAA3H (cosmid)
Promoters: Promoter none
Construction: pBR322, lambdagal+(EcoRI/HindIII, galE'TK-cos R/L)
Marker(s):ampR,galS
Construct size (kb): 11.5
Features: insert detection: galE'TK
marker(s): ampR, galS
promoter: none
replicon: pMB1
terminator: none
enhancer: none
Applications
vector for constructing genomic libraries
vector permitting positive selection for inserts
Comments
The galE'TK genes are carried on a HindIII/HpaI fragment of approximately 3.0 kb. The galE gene is truncated at the HindIII site. Expression of the gal sequences are regulated by a promoter located between the BamHI and HindIII sites in the tet gene.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Vector for positive galactose-resistance selection of DNA cloned into the HindIII site.
Derived from pAA3.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Ahmed A. Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli. Gene 28: 37-43, 1984. PubMed: 6329914

Shipped
freeze-dried
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