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HIBBC63

規格:

價格:

編號:B230021

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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產品名稱

HIBBC63

商品貨號

B230021

Designations

HIBBC63

GenBank Number

M25756

T08333

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 5.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCGR*
Gene name: secretogranin II
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M25756; EST: EST06224
Nucleotides 1-390 of the insert correspond to
nucleotides 721-1110 of M25756 with 98% identity.
DNA Seq. Acc.: T08333; EST: EST06224; Primer: M13 Reverse;
THC id: THC85513

Insert lengths(kb): 1.700000047683716

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: secretogranin II [SCGR*]

Insert Size (kb)

1.700

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.7, EcoRI--5.0.

Based on the alignment with GenBank accession M25756 (2336 nt, CDS 63-1916), this clone appears to contain part of the coding sequence.

The cDNAs (>95%) are cloned directionally with the 5' end adjacent to the M13 reverse primer and HindIII.

References

Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585

Mark D Adams, personal communication

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