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p416 GAL1 in E. coli

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編號:B230066

品牌:Mingzhoubio

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產品名稱	p416 GAL1 in E. coli
商品貨號	B230066
Designations	p416 GAL1 in E. coli
Depositors	M Funk
Biosafety Level	1
Vector Information	Size (kb): 5.5840001106262210 Vector: p416 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS416, GAL1 promoter Marker(s):URA3,ampR Construct size (kb): 5.584000110626221 Features: marker(s): URA3 marker(s): ampR promoter for expression: GAL1 MCS: XhoI...XbaI terminator: CYC1 centromere: CEN6/ARSH4
Applications	YE-type (episomal) shuttle vector YX-type (expression) shuttle vector vector with low copy number
Comments	Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 0.5; EcoRI--5.6; KpnI--5.6. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736
Related Products	component of:ATCC 87670
Shipped	frozen

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