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pGX2907 [GX3305, GX3320]
pGX2907 [GX3305, GX3320]
規格:
價格:
編號:B230168
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGX2907 [GX3305, GX3320]
商品貨號
B230168
Designations
pGX2907 [GX3305, GX3320]
Species
Staphylococcus aureus Rosenbach
培養基
Depositors
Genex Corp.
Vector
Construct size (kb): 0.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGX284
Intact vector size: 2.150
Type of vector: plasmid
Vector end: EcoRV
Vector end: EcoRV
Cloning sites: EcoRV
Polylinker sites:
Construction: pBR322, pC194, bacteriophage lambda, Bacillus subtilis
Host range: Bacillus subtilis; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR
replicon: pMB1
terminator: lambda
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Staphylococcus aureus
Gene symbol: pra
Gene name: protein A
Contains complete coding sequence?: U
Genome: Staphylococcus aureus
Gene symbol: pra
Gene name: protein A
Contains complete coding sequence?: U
Strain: Cowan I
Type of DNA: genomic
Insert end: EcoRV
Insert end: EcoRV
Insert size (kb): 2.15
Cross references:
Insert lengths(kb): 2.150000095367432
Gene product: protein A [pra]
Insert Size (kb)
2.150
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV--8.8, 2.15; PstI--5.6, 3.1, 2.3; HindIII--9.4, 1.6; PvuI--8.8, 2.15; BamHI--uncut.
This construct is available in two forms: in Escherichia coli as the plasmid (ATCC 39344) and integrated into the Bacillus chromosomal DNA (ATCC 39345).
The depositor suggests streaking for single colonies on L broth + 1.5% agar + 100 ug/ml ampicillin + 5 ug/ml chloramphenicol and using a single colony.
The vector contains the replicon and beta-lactamase gene from pBR322, a galactokinase gene, a CAT gene from pC194, and a transcription terminator from bacteriophage lambda. It also contains chromosomal sequences from Bacillus subtilis for integration.
References

Fahnestock SR. Production of protein A. US Patent 4,617,266 dated Oct 14 1986

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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