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GMCSF [pCSF-1]
GMCSF [pCSF-1]
規格:
價格:
編號:B230181
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
GMCSF [pCSF-1]
商品貨號
B230181
Designations
GMCSF [pCSF-1]
Species
Homo sapiens, human
培養基
Depositors
Genetics Institute, Inc., GG Wong, Genetics Institute, Inc.
Applications
in another host, produces protein colony stimulating factor 2 (granulocyte-macrophage)
Vector
Construct size (kb): 9.75
Name of vector: p91023(B)
Intact vector size: 8.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: SV40
marker(s): tetR
promoter: Ad2 major late
replicon: pMB1, SV40
terminator: SV40
Insert
DNA: cDNA
Genome: human
Gene symbol: CSF2
Genomic copy number: unique
Gene name: colony stimulating factor 2 (granulocyte-macrophage)
Contains complete coding sequence?: U
Chromosome: 5; Localization: 5 q23-q31
Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066)
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.8
Insert lengths(kb): 0.80
Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066)
Gene product: colony stimulating factor 2 (granulocyte-macrophage) [CSF2]
Target Gene: colony stimulating factor 2 (granulocyte-macrophage)
Insert Size (kb)
0.800
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.0, 0.81; HindIII--4.8, 1.6, 1.3, 0.6.
This clone may be most reliably recovered by streaking on tetracycline-containing plates, picking single colonies, and doing a miniprep.
Contains 8 bp 5' untranslated; ORF of 432 nucleotides encoding 144 amino acids (complete coding sequence), and 3' untranslated sequences.
The vector allows expression in COS cells. The insert contains PstI, BglI, NcoI and AhaIII sites.
References

Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987

Rajagopalan LE, Malter JS. Turnover and translation of in vitro synthesized messenger RNAs in transfected, normal cells. J. Biol. Chem. 271: 19871-19876, 1996. PubMed: 8702698

Wong GG, et al. Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228: 810-815, 1985. PubMed: 3923623

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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