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pPLc28-HIIF52 [HIIF-E]
pPLc28-HIIF52 [HIIF-E]
規(guī)格:
價格:
編號:B230556
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pPLc28-HIIF52 [HIIF-E]
商品貨號
B230556
Designations
pPLc28-HIIF52 [HIIF-E]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Biogen N.V.
Applications
produces protein interferon gamma
Vector
Construct size (kb): 3.700000047683716
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pPLc28
Intact vector size: 2.720
Type of vector: plasmid
Cloning sites: EcoRI BamHI HindIII
Polylinker sites: EcoRI BamHI HindIII
Construction: pBR322, lambda
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: lambdaPL
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFNG
Genomic copy number: unique
Gene name: interferon, gamma
Contains complete coding sequence?: U
Chromosome: 12; Localization: 12 q24.1
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.1
Cross references:
Insert lengths(kb): 1.100000023841858
Gene product: interferon gamma [IFNG]
Target Gene: interferon gamma
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): ClaI/HindIII--2.9, 1.0; BamHI--3.9; EcoRI--uncut; PstI--3.9; BglI--2.1, 1.8.
Absence of a transcription terminator 3' to the insert may cause instability of the plasmid after induction, due to interference with plasmid replication.
When induced at 42C, interferon gamma (insoluble) can be produced to a level up to 14.7 percent of the total cellular protein.
Consists of pPLc28 with the lambda PL promoter, a manipulated ribosome binding site from the E. coli trp attenuator, an engineered ATGTGTTAC sequence, and a sequence corresponding to nt 156-1170 of the sequence record.
References

Simons G, et al. High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. Gene 28: 55-64, 1984. PubMed: 6234201

Fiers WC, Allet B. DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields. US Patent 5,004,689 dated Apr 2 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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