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HSE26.1

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價格:

編號:B230699

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

HSE26.1

商品貨號

B230699

Designations

HSE26.1

GenBank Number

J04071

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 3.799999952316284

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIBI24
Intact vector size: 2.878
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Construction: pEMBL
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GZMB
Other gene symbols: CTLA1; CSPB; CCPI; CGL-1
Genomic copy number: unique
Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated
serine esterase 1)
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q11.2
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: J04071

Insert lengths(kb): 0.8999999761581421

Tissue: T lymphocyte, activated

Gene product: cytotoxic serine protease B [CCPI]

Insert Size (kb)

0.900

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95.

Contains the complete coding sequence (nt 17-760).

The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72.

Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0.

References

Trapani JA, et al. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA 85: 6924-6928, 1988. PubMed: 3261871

Klein JL, et al. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes. Genomics 5: 110-117, 1989. PubMed: 2788607

B206944：HSE26.1

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