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pH60 [TRGJ1-2]
pH60 [TRGJ1-2]
規(guī)格:
價格:
編號:B230770
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pH60 [TRGJ1-2]
商品貨號
B230770
Designations
pH60 [TRGJ1-2]
GenBank Number

X03434

培養(yǎng)基
Species
Homo sapiens, human
Depositors
M Lefranc, TH Rabbitts
Vector
Construct size (kb): 3.299999952316284
Insert
DNA: genomic
Insert lengths(kb): 0.699999988079071
Gene product: T cell receptor, gamma cluster [TCRG]
Alleles: J2, G1, G2, J1
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 0.7; EcoRI--3.3; HindIII--3.3.
The frequencies of the TRGJ2 alleles, which have been defined by the polymorphic HindIII(*A) and EcoRI(*B) restriction fragments, have been calculated in four different populations.
The constant bands detected by HindIII and EcoRI are from TCRGJ1; the polymorphic bands are from TCRGJ2.
pH60 can detect all the TCRG rearrangements to J1 and J2 when hybridized to BamHI, EcoRI and HindIII digests.
pH60 can detect all the TCRG rearrangements to JP1, JP and JP2 when hybridized to KpnI digests.
Subcloned from M13H60 (ATCC 59586, 59587). Contains the TCRGJ1 gene.
References

Lefranc MP, Rabbitts TH. Two tandemly organized human genes encoding the T-cell gamma constant-region sequences show multiple rearrangement in difference T-cell types. Nature 316: 464-466, 1985. PubMed: 2991773

Lefranc MP, et al. Rearrangement of two distinct T-cell gamma-chain variable-region genes in human DNA. Nature 319: 420-422, 1986. PubMed: 3003579

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Lefranc MP, et al. Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene gamma. Proc. Natl. Acad. Sci. USA 83: 9596-9600, 1986. PubMed: 2879283

Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332

Huck S, Lefranc MP. Rearrangements to the JP1, JP and JP2 segments in the human T-cell rearranging gamma gene (TRGgamma)locus. FEBS Lett. 224: 291-296, 1987. PubMed: 2961609

Buresi C, et al. Exon duplication and triplication in the human T-cell receptor gamma constant region genes and RFLP in French, Lebanese, Tunisian, and Black African populations. Immunogenetics 29: 161-172, 1989. PubMed: 2564370

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Sturm E, et al. Coordinated Vgamma and Vdelta gene segment rearrangements in human T cell receptor gamma/delta+ lymphocytes. Eur. J. Immunol. 19: 1261-1265, 1989. PubMed: 2547624

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713

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