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pCSF25/5
pCSF25/5
規格:
價格:
編號:B230996
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCSF25/5
商品貨號
B230996
Designations
pCSF25/5
GenBank Number

M20021

培養基
Species
chloris striate mosaic geminivirus (CSMV)
Depositors
BA Morris
Vector
Construct size (kb): 5.40
Insert
DNA: Genomic
Insert lengths (kb): 2.75
Tissue: DNA from infected grass (Dactyloctenium australe)
Insert Size (kb)
2.75
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 2.7; KpnI--4.9, 0.5; PstI--3.15, 1.4, 0.87; SacI--5.4.

The insert contains the following restriction sites (approximate kb from a BamHI site): EcoRI--2.17; KpnI--0.23, 0.69; PstI--0.42, 1.32; SacI--0.94.

Features of the virus occupy the following positions: LIR--(2-233); SIR--(1376-1579); ORF 33.1--(2750-1867); ORF 15.8--(1987-1580); ORF 26.8--(658-1335); ORF 14.5--(234-1375).

Constructed by linearizing the viral ds DNA at the unique BamHI site (nt 351) within the ORF encoding a 14.5 kDa protein.
Classification
Geminivirus
References

Andersen MT, et al. Nucleotide sequence of the Geminivirus chloris striate mosaic virus. Virology 164: 443-449, 1988. PubMed: 3369088

Morris BA, et al. Plant Mol. Biol. 11: 759-803, 1988.

Bret A Morris, personal communication

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