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HHCPO72

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編號:B231026

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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產品名稱

HHCPO72

商品貨號

B231026

Designations

HHCPO72

GenBank Number

M79177

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 7.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FASN
Gene name: fatty acid synthetase
Contains complete coding sequence?: U
Chromosome: 17
Genome: Homo sapiens
Gene symbol: D0S2143E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.2
Cross references: DNA Seq. Acc.: M79177; EST: EST01325; Primer: M13 Forward;
THC id: THC129775

Insert lengths(kb): 4.199999809265137

Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted

Gene product: fatty acid synthetase [FASN]

Insert Size (kb)

4.200

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 3.0; BamHI--5.0, 2.0, 0.3; PvuII--2.9, 2.7, 0.5, 0.3, 0.27, 0.23.

EST01325 matches GenBank accession RATFAS with 79.79% identity over the sequence range: 101-3.

This sequence record is for rat fatty acid synthetase.

References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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