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Human herpesvirus 3
Human herpesvirus 3
規(guī)格:
價(jià)格:
編號(hào):B231225
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human herpesvirus 3
商品貨號(hào)
B231225
Classification
Herpesviridae, Varicellovirus
Deposited As
Varicella-Zoster
培養(yǎng)基
Agent
Human herpesvirus 3
Strain
Ellen
Common Name
Varicella zoster virus
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
Liquid nitrogen vapor phase
Comments
VR-1367™ is a mycoplasma-free item derived at ATCC® from VR-586™ by three passages in the presence of a mycoplasma  removal agent.
Effect on Host
CPE, syncytial rounding and sloughing
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: This product is produced by co-cultivation of virus with fresh host cells. Seed culture vessels at 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 4-7 days
Name of Depositor
EI Rosanoff, Wyeth Laboratories, Inc.
Special Collection
NCRR Contract
Source
Vesicular fluid from child with chickenpox, Georgia. Derived from VR-586™. Treated to remove Mycoplasma hominis at ATCC®.
References

Brunell PA, Casey HL. Crude tissue culture antigen for determination of Varicella-Zoster complement fixing antibody. Public Health Rep. 79: 839-842, 1964. PubMed: 14213793

Brunell PA. Transmission of chickenpox in a school setting prior to the observed exanthem. Am. J. Dis. Child. 143: 1451-1452, 1989. PubMed: 2556025

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