Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
This product is a mycoplasma free preparation derived from ATCC VR-489.
Product Format
frozen
Storage Conditions
Store at ?-70°C or colder
Comments
This product is a mycoplasma free preparation derived from ATCC VR-489.
Effect on Host
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1. Adsorb 1 hour at 33.0°C in a humidified, 5% CO2 atmosphere with continuous rocking. End adsorption by adding virus growth medium.
Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 3-7 days with rocking, until CPE are well advanced in 100% of the culture.
Name of Depositor
VV Hamparian, RM Conant
Source
Presumed from human throat washings; derived from ATCC VR-489 following treatment to remove mycoplasma contamination.
