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pODC821
pODC821
規(guī)格:
價格:
編號:B231742
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pODC821
商品貨號
B231742
Designations
pODC821
GenBank Number

X07944

培養(yǎng)基
Species
Rattus norvegicus, rat
Depositors
CT Van Oostrom, H van Steeg
Applications
in suitable host, produces protein ornithine decarboxylase 1
Vector
Construct size (kb): 10.44999980926514
Insert
DNA: genomic
Insert lengths(kb): 7.776000022888184
Tissue: liver
Gene product: ornithine decarboxylase 1 [ODC1]
Target Gene: ornithine decarboxylase 1
Insert Size (kb)
7.776
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 2.7; PstI--4.2, 3.8, 1.0, 0.65, 0.5, 0.3; EcoRI--5.3, 5.2; HindIII--4.9, 2.6, 2.4, 0.6; SalI--10.5.
The insert contains the following restriction sites (nt from the 5' end): PstI--1170 (exon 1), 1603 (intron 1), 5820 (exon 10), 6892, 7523 (exon 12); HindIII--2313 (intron 1), 4935 (exon 8), 7206 (exon 12); EcoRI--5207 (intron 8).
Contains the complete gene (12 exons) with 5' and 3' flanking sequences.
When the insert is cloned into pSV2neo and transfected into enzyme-deficient CHO cells, the enzyme is synthesized and is inducible by insulin or tetradecanoyl phorbol acetate.
References

van Steeg H, et al. Cloning and functional analysis of the rat ornithine decarboxylase-encoding gene. Gene 93: 249-256, 1990. PubMed: 2227438

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