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p144-2
p144-2
規格:
價格:
編號:B231770
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p144-2
商品貨號
B231770
Designations
p144-2
Species
Homo sapiens, human
培養基
Depositors
HG Zachau, W Pargent
Applications
The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic.
Vector
Construct size (kb): 3.349999904632568
Insert
DNA: genomic
Insert lengths(kb): 0.3499999940395355
Tissue: placenta St
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb)
0.350
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.5, 0.3; PvuI--2.2, 1.2; PvuI/EcoRI--1.7, 1.1, 0.6; PstI--3.4; PvuII/XbaI--2.5, 0.3, 0.3, 0.2.
Detects sequences from the Op (between O12 and O13) and Od (between O2 and O2) regions.
Does not cross-hybridize to the W region.
The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic.
Contains the SalI fragment from m144-2 which had been generated by adding SalI linkers to an RsaI fragment.
During recloning, the SalI end 150 bp from the vector PvuII site was destroyed. Neither KpnI, ApaI nor XhoI cut at this position. The insert is not readily excised, but can be amplified with standard M13 sequencing (forward and reverse) primers.
References

Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350

Pohlenz HD, et al. The human V(k) locus: characterization of extended immunoglobulin gene regions by cosmid cloning. J. Mol. Biol. 193: 241-253, 1987. PubMed: 3037085

Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917

Hans G Zachau, personal communication

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