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lambdaSP19
lambdaSP19
規(guī)格:
價格:
編號:B231819
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
lambdaSP19
商品貨號
B231819
Designations
lambdaSP19
Species
Homo sapiens, human
培養(yǎng)基
Depositors
M Lefranc
Vector
Construct size (kb): 44.09999847412109
Insert
DNA: genomic
Insert lengths(kb): 14.60000038146973
Tissue: T-lymphocyte SP-F7 cell line
Gene product: T cell receptor, gamma, variable region V5 [TCRGC1]
Insert Size (kb)
14.600
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--19.0, 13.0, 6.4, 4.6; BglII--27.0, 8.0, 7.5; EcoRI--23.0, 10.0, 3.4, 2.8, 2.5; BamHI--22.0, 20.0; HindIII--22.0, 11.5 (doublet); PvuI--20.0, 15.0, 8.5; SacI--20.0, 9.4, 8.5, 4.4.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--1.65, 5.4, 5.8, 12.1; EcoRI--3.05, 6.0, 8.3, 11.0; HindIII--3.15, 3.6, 7.8, 12.5; BamHI--14.6.
The 3' end of this insert is the naturally occurring BamHI site in C1 exon 1.
Contains germline V5, germline V4, the 5' end of C1 exon 1, and an unusual rearrangement abutting the V3 recombination signal to J1 in an inverted orientation.
lambdaSP14 (ATCC 65836, 65837) and lambdaSP19 (ATCC 65838, 65839) are end clones from the same inversion (47 kb) event.
References

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

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