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YEp426 [pRB687]
YEp426 [pRB687]
規(guī)格:
貨期:
編號(hào):B231874
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
YEp426 [pRB687]
商品貨號(hào)
B231874
Designations
YEp426 [pRB687]
Depositors
D Botstein
Biosafety Level
1
Vector Information
Size (kb): 11.1999998092651400
Vector: YEp426 (plasmid)
Construction: YEp425, pBR322
Marker(s):ampR,LYS2
Construct size (kb): 11.19999980926514
Features: marker(s): ampR, LYS2
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.4, 3.8; BamHI/HindIII--7.0, 3.8, 1.4; ClaI/SalI--6.8, 6.0.
YE-type shuttle vector.
The order of the major features in this plasmid is: ClaI site - LYS2 - SalI site - ori(2 micron) - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185

Shipped
freeze-dried
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