3d动漫啪啪精品一区二区中文字幕,麻豆回家视频区一区二,午夜偷拍福利

VMM17

規格:

價格:

編號:B231882

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

VMM17

商品貨號

B231882

Organism

Homo sapiens, human

Tissue

Melanoma, Lymph Node Metastasis

培養基

Cell Type

Melanocyte

Product Format

frozen

Morphology

Epithelial-like

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Melanoma, Stage IIIB; malignant

Age

Gender

Female

Ethnicity

Caucasian

Applications

Drug screening
Development of targeted therapy
Development of combination therapy
Tumor vaccine development

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia

Clinical Data

Primary Site: unknown; Metastatic Site: Lymph Node, Right Groin

Antigen Expression

Positives: MAGE-A1, MAGE-A3

HLA Typing

A2,?[A2 OR A32],B65/14(B*14),B45,Cw8,Cw16 (HLA: test not performed. 2nd A Allele possible but not necessary; may have lost A33/34 allele.)

Comments

NRAS: wt
CDKN2A Mutation: R80
BRAF Mutation: V600E

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes are for a T-75 flask; Adjust accordingly

Remove and discard the cell culture medium from the flask.
Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove.
Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC.
Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the flask. Check the cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting.
Subculture as necessary.
Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing.
Repeat when cells reach confluence.

Cryopreservation

Fetal bovine serum, 90%; DMSO, 10%

Culture Conditions

Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO₂ ), 5%

STR Profile

Amelogenin: X
D5S818: 12
D13S317: 11,12
D7S820: 9,10
D16S539: 12
vWA: 14,17
TH01: 9.3
TPOX: 8,11
CSF1PO: 10,12

Sterility Tests

Pass

Name of Depositor

Craig L. Slingluff, Jr. M.D.

Passage History

Unknown. 2 passages from 12/27/1996 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1994.

Year of Origin

1994

References

Slingluff C, et al. Melanomas with concordant loss of multiple melanocytic differentiation proteins: immune escape that may be overcome by targeting unique or undefined antigens. Cancer Immunol. Immunother. 48:661-672, 2000. PubMed: 10752474

Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701

Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479