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Human herpesvirus 5
Human herpesvirus 5
規(guī)格:
價(jià)格:
編號(hào):B231930
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human herpesvirus 5
商品貨號(hào)
B231930
Classification
Herpesviridae, Cytomegalovirus
Deposited As
Cytomegalovirus
培養(yǎng)基
Agent
Human herpesvirus 5
Strain
Davis
Common Name
Human cytomegalovirus
Applications
Sexually Transmitted Disease Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Liver biopsy, 3-month-old girl with microencephaly and other symptoms resembling congenital toxoplasmosis
Product Format
frozen 1.0 mL per vial
Storage Conditions
Vapor phase of liquid nitrogen
Images
VR-807 Micrograph of Host Effects
Comments
Virus primarily cell-associated. For each passage, perform freeze-thaws to facilitate viral release. A long (10-11 day) incubation is required for initial passage of frozen stocks. Add contents of ampule to a 80-90% monolayer of cells. For preservation, add FBS to virus culture to produce a final 20% FBS concentration.
Effect on Host
CPE, focal or generalized cytopathology with rounding, cytomegaly, and intranuclear inclusions
Recommended Host
MRC-5 cells (ATCC® CCL-171™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
T Weller
Special Collection
NCRR Contract
Source
Liver biopsy, 3-month-old girl with microencephaly, and other symptoms resembling congenital toxoplasmosis
References

Weller TH, et al. Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proc. Soc. Exp. Biol. Med. 94: 4-12, 1957. PubMed: 13400856

Weller TH, et al. Serologic differentiation of viruses responsible for cytomegalic inclusion disease. Virology 12: 130-132, 1960. PubMed: 13843861

Cross References

Nucleotide (GenBank) : JX512198.1 Human herpesvirus 5 strain Davis, complete genome

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