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Leptomonas pulexsimulantis Beard et al.
Leptomonas pulexsimulantis Beard et al.
規格:
價格:
編號:B232071
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Leptomonas pulexsimulantis Beard et al.
商品貨號
B232071
Application
In vitro growth characterization and host parasite relationship
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

培養基
Isolation
adult dog flea, Pulex simulans, Alachua Co., FL, 1985
Product Format
frozen
Type Strain
yes
Comments
In vitro growth characterization and host parasite relationship
Medium
ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor
CB Beard
Year of Origin
1985
References

Beard CB, et al. In vitro growth characterization and host-parasite relationship of Leptomonas pulexsimulantis n. sp., a trypanosomatid flagellate of the flea Pulex simulans. J. Parasitol. 75: 658-668, 1989.

type strain

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