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Hepatitis A virus
Hepatitis A virus
規格:
貨期:
編號:B232201
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hepatitis A virus
商品貨號
B232201
Classification
Picornaviridae, Hepatovirus
Agent
Hepatitis A virus
Strain
HM 175 (clone 1)
Applications
Enteric Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Effect on Host
No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration
Recommended Host
FRhK4 (ATCC® CRL-1688™)
Growth Conditions
Temperature: 34.5°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34.5°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-28 days
Name of Depositor
RH Purcell, National Institute of Allergy and Infectious Diseases; ATCC
Special Collection
NCRR Contract
Source
Human stool specimens from an outbreak of the virus, Australia.
References

Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685

Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985

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