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p5T4-1-27
p5T4-1-27
規(guī)格:
價(jià)格:
編號(hào):B232220
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
p5T4-1-27
商品貨號(hào)
B232220
Designations
p5T4-1-27
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 51.09999847412109
Insert
DNA: genomic
Insert lengths(kb): 48.79999923706055
Tissue: lymphocyte
Gene product: excision repair cross-complementing rodent repair deficiency, complementation group 2(DNA excision repair protein ERCC2) [ERCC2]
Insert Size (kb)
48.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb, * = doublet): EcoRI--10.5, 10.1, 8.8, 5.4, 4.6*, 2.9, 2.5*, 1.0; XhoI--20.8, 10.7, 10.3; SalI--20.8*; BamHI--10.3*, 10.1*, 6.6, 3.5, 2.6, 1.8, 1.0; PstI--10.5, 6.2, 4.4*, 3.1 + more.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The insert contains Alu repeats, but the gene is single copy.
p5T4-1-27 was isolated from a hamster line transformed with human lymphocyte/hamster hybrid DNA and pSV2gpt. The insert is a human gene with flanking human, pSV2gpt, and hamster sequences.
p14B1 is 2.3 kb and contains ColE1 and ampR of pBR327, lambda cos sites, and a BamHI cloning site flanked by EcoRI sites 6 bp on either side.
References

Weber CA, et al. Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells. Mol. Cell. Biol. 8: 1137-1146, 1988. PubMed: 2835663

Christine A Weber, personal communication

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