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pSM-1 [UTR-00282, WI-8985]
pSM-1 [UTR-00282, WI-8985]
規格:
價格:
編號:B232282
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSM-1 [UTR-00282, WI-8985]
商品貨號
B232282
Designations
pSM-1 [UTR-00282, WI-8985]
GenBank Number

X02744

培養基
Species
Homo sapiens, human
Applications
A 2.0 kb BamHI fragment was used to detect the RFLP.
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.599999904632568
Tissue: HuT-102 cell line (adult T-cell leukemia)
Gene product: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)(platelet-derived growth factor, beta polypeptide) [SIS]
Alleles: B1, B2, B1, B2
Insert Size (kb)
2.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.1, 0.68; SalI--5.8; HindIII--5.8; PstI--4.6, 1.35; BglII--5.8.
The insert includes 2498 bp followed by 92 adenylates. The insert is located downstream from a SV40 enhancer, early promoter and 16S and 19S mRNA splice sites, and upstream from SV40 poly(A) signal.
The insert contains a BamHI site. The 2.0 kb BamHI fragment contains all the coding sequences of the mRNA, plus some pcD sequence at the 5' end.
A 2.0 kb BamHI fragment was used to detect the RFLP.
References

Clarke MF, et al. Transformation of NIH 3T3 cells by a human c-sis cDNA clone. Nature 308: 464-467, 1984. PubMed: 6323994

Josephs SF, et al. Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor. Science 225: 636-639, 1984. PubMed: 6740330

Ratner L, et al. Nucleotide sequence of transforming human c-sis cDNA clones with homology to platelet-derived growth factor. Nucleic Acids Res. 13: 5007-5018, 1985. PubMed: 2991848

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