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Human respiratory syncytial virus
Human respiratory syncytial virus
規(guī)格:
價(jià)格:
編號(hào):B232599
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human respiratory syncytial virus
商品貨號(hào)
B232599
Classification
Paramyxoviridae, Pneumovirus
Deposited As
Respiratory syncytial virus
培養(yǎng)基
Agent
Human respiratory syncytial virus
Strain
A2
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
Human respiratory syncytial virus ATCC VR-1540 images
Comments
VR-1540 was prepared from ATCC VR-1302; by growth in the presence of neutralizing serum to remove contaminating Human adenovirus 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HEp-2 (ATCC CCL-23)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
L Potash, Flow Laboratories, Inc.; ATCC
Special Collection
NCRR Contract
Source
Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961
References

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.

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