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pAV1

規格:

價格:

編號:B232615

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAV1

商品貨號

B232615

Designations

pAV1

Species

Adeno-associated virus 2

培養基

Depositors

CA Laughlin

Applications

Contains the complete viral genome for use in site-specific mutagenesis.

Vector

Construct size (kb): 9.0

Insert

DNA: genomic

Insert lengths(kb): 4.7

Gene product: human adeno-associated virus type 2

Insert Size (kb)

4.700

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BglII--4.7, 4.0; HindIII--6.6, 1.9; EcoRI--3.9, 2.8, 1.9.

pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted).

Contains the complete viral genome for use in site-specific mutagenesis. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles.

Classification

Parvoviridae, Dependovirus, Adeno-associated virus-2

References

Laughlin CA, et al. Cloning of infectious adeno-associated virus genomes in bacterial plasmids. Gene 23: 65-73, 1983. PubMed: 6352411

B215057：PAV16

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