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pJJ217
pJJ217
規格:
價格:
編號:B232815
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pJJ217
商品貨號
B232815
Designations
pJJ217
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養基
Depositors
L Prakash
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.764000058174133
Gene product: imidazoleglycerol-phosphate dehydratase [HIS3]
Insert Size (kb)
1.764
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 1.8; PvuII--2.4, 2.0; EcoRI--4.4.
BglII and HindIII cut multiple times within the reading frame.
A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions.
HIS3 is in an orientation opposite to lacZ'.
The insert was subcloned from pSZ515.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072

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