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S-ENDO3.3
S-ENDO3.3
規(guī)格:
價(jià)格:
編號(hào):B232871
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
S-ENDO3.3
商品貨號(hào)
B232871
Designations
S-ENDO3.3
GenBank Number

X72012

培養(yǎng)基
Species
Homo sapiens, human
Depositors
C Bernabeu
Vector
Construct size (kb): 5.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC13
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Construction: pBR322, Escherichia coli lacZalpha, M13mp11
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ENG
Other gene symbols: HHT1; ORW1; END
Gene name: endoglin (Osler-Rendu-Weber syndrome 1)
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: HL-60 cell line, PMA-treated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.1
Cross references: DNA Seq. Acc.: X72012
Nucleotides 1-3073 of the insert correspond to
nucleotides 1-3073 of X72012.
Insert lengths(kb): 3.099999904632568
Tissue: HL-60 cell line, PMA-treated
Gene product: endoglin (Osler-Rendu-Weber syndrome 1)(Endoglin) [END]
Insert Size (kb)
3.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; HindIII--5.9; BamHI/MluI--3.0, 2.3, 0.58; BamHI--3.0, 2.9; PstI--4.4, 0.87, 0.68.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.7; BglI--3.0; PstI--0.33, 1.04, 1.28.
Encodes the S-isoform with a monomeric molecular weight of 85 kDa. This form, which is not the predominant one, includes an exon of 135 bp.
References

Bellon T, et al. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23: 2340-2345, 1993. PubMed: 8370410

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