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Bb-59 [T66]

規格:

價格:

編號:B232893

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Bb-59 [T66]

商品貨號

B232893

Designations

Bb-59 [T66]

Species

Mus musculus, mouse

培養基

Depositors

LM Silver

Vector

Construct size (kb): 7.400000095367432

Insert

DNA: genomic

Insert lengths(kb): 4.5

Tissue: liver

Gene product: DNA segment, Chr 17, Lehrach-66 [D17Leh66]

Alleles: A-a, B-a, B-b, B-b, C-a, C-x, E-b, E-b, E-b, C-b1, C-b2, C-b2, C-g1, C-g3, C-g4, D-b1, D-b1, D-b1, D-b2, D-b2, D-b2, D-g1, D-g2, E-a

Insert Size (kb)

4.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 2.85; BamHI--4.2, 2.1, 1.55; PstI--3.8, 1.6, 1.3, 0.74; HindIII--3.8, 3.1, 1.1.

Mapped by probing DNA from mice with different partial t haplotypes.

The 1.5 kb XhoI/EcoRI portion of the insert hybridizes specifically to the beta subfamily of elements present at the mouse D17Leh66 complex locus.

Isolated from a genomic library constructed from MboI partially digested, size fractionated (30-50 kb) DNA from a male mouse with a t complex genotype of t(wlub1)/t(w5).

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Schimenti J, et al. An unstable family of large DNA elements in the center of the mouse t complex. J. Mol. Biol. 194: 583-594, 1987. PubMed: 2821263

Lee M Silver, personal communication

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