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Gallid herpesvirus 2

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貨期:

編號:B233236

品牌:Mingzhoubio

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定量菌液

DNA

RNA

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產品名稱

Gallid herpesvirus 2

商品貨號

B233236

Classification

Herpesviridae, Mardivirus

Agent

Gallid herpesvirus 2

Strain

648A

Applications

Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.

Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium.

With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype.

Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines.

Positive for Mycoplasma

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Isolated from chicken blood lymphocytes

Product Format

frozen

Storage Conditions

?Vapor phase of liquid Nitrogen

Comments

Set up cells 24 to 48 hours in advance. Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium. Adsorption is not required. Incubate 4-7 days at 38° C in a humidified 5% CO2 atmosphere. Harvest by trypsinizaion of infected cell culture. Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.

Widely recognized representative of the MDV vv+ pathotype. With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype. Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines. Also infects turkeys and quail. The virus is highly cell associated and must be propagated and stored as viable infected cells. A stock of infected cells at high density (107/ml) typically has an infectivity titer of about 106 PFU/ml.

Effect on Host

Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes. In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.

Effects: ?Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes.? In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.

Recommended Host

Recommended Host: ?Duck or chick embryo fibroblast cultures (e. g. ATCC^? CRL-1590™)

Growth Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 38.0°C

Duration: 4-7 days required for infection. Virus is highly cell associated and must be preserved in the form of viable cryopreserved cells.

Cryopreservation

Freeze medium: Culture medium containing 10% serum and 10% DMSO. Controlled rate freezing is recommended.

Storage temperature: Liquid Nitrogen

Name of Depositor

RL Witter

Special Collection

ATCC

Source

Isolated from chicken blood lymphocytes

References

Witter RL. Increased virulence of Marek's disease field isolates. Avian Dis. 41: 149-163, 1997. PubMed: 9087332

Dudnikova E, et al. Evaluation of Marek's disease field isolates by the "best fit" pathotyping assay. Avian Pathology 36(2):135-143, 2007.

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