Restriction digests of the clone give the following sizes (kb): EcoRI--2.6; HindIII--2.6; XmnI--2.6.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site and rrnB terminators downstream.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
