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pT109luc Plasmid in Escherichia coli
pT109luc Plasmid in Escherichia coli
規格:
價格:
編號:B233430
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pT109luc Plasmid in Escherichia coli
商品貨號
B233430
Designations
pT109luc Plasmid in Escherichia coli
Depositors
SK Nordeen
培養基
Biosafety Level
1
Vector Information
Size (kb): 6.09
Vector: pT109luc (plasmid)
Promoters: Promoter HSV TK
Construction: pXP2, herpes simplex virus TK promoter
Marker(s):ampR
Construct size (kb): 6.09
Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: none
enhancer: none
Applications
 expression vector
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
This contains bp -109 to +52 of the herpes simplex tk promoter; both GC + CCAAT are present.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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