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pCNV220
pCNV220
規格:
價格:
編號:B233667
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCNV220
商品貨號
B233667
Designations
pCNV220
GenBank Number

M25270

培養基
Species
Cucumber necrosis virus (CNV)
Depositors
DM Rochon
Applications
contains sequence genome, 90%+ complete
Vector
Construct size (kb): 7.5
Insert
DNA: cDNA
Insert lengths(kb): 4.699999809265137
Gene product: genome, 90%+ complete
Target Gene: genome, 90%+ complete
Insert Size (kb)
4.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.6, 3.0; HindIII--3.5, 1.4, 1.3, 1.2; BglI--2.7, 1.7, 1.5 (doublet).
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--2.66, 4.29; BglII--3.36; EcoRI--2.14; EcoRV--1.07, 1.49, 1.57, 3.19, 4.03; HindIII--0.62, 1.80, 3.22; KpnI--3.89; NdeI--0.64, 1.58, 3.52, 3.63; PvuII--2.49, 3.33.
Contains entire coding sequence plus 20 nucleotides of 5' noncoding sequence.
Virus was isolated from cucumber, Cucumis sativus (cultivar strain 8).
Classification
Tombusvirus
References

Rochon DM, Tremaine JH. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169: 251-259, 1989. PubMed: 2705296

D'Ann M Rochon, personal communication

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